Publicações

Evaluation of pregnancy rate using two soft embryo transfer catheters

Juliana Polisseni¹, Carolina M de Assunção¹, Fernanda Polisseni¹, Josélio V Rosa¹, Jociane V S Oliveira¹, Larissa M Coutinho¹, João P J Caetano¹
JBRA Assist.Reprod. 2016;20 (3):168-193

POSTER PRESENTATIONS
doi:10.5935/1518-0557.20160036
Abstracts of the 20th Annual Congress of the SBRA, Belo Horizonte/MG 14-17 September 2016

OBJECTIVE: 
We compared the efficacy of two different soft catheters on total pregnancy rates in patients undergoing ICSI.

METHODS:
A retrospective study was performed at Clinical of Reproductive Medicine in Zona of Mata Mineira, from April 2015 to April 2016. A total of 158 patients of ICSI cycle undergoing fresh embryo transfer (ET) were evaluated: Friedman IVF catheter (N=125) and Sydney IVF catheter (N=39). Chi-square test (P<0.05) was used to compare the effect of the catheters on the pregnancy rates. Difference between mean of age and number of embryo transfer of groups were evaluated Student’s t test (P<0.05) as mean±SEM.

RESULTS:
The mean age of the patients in the Friedman IVF catheter group was 35.31±0.46 (range: 18-47) and in the Sydney IVF catheter group was 35.18±0.94 (range: 22-50). For number of embryo transfer, the result of Friedman IVF catheter was 2.28±0.07 and Sydney IVF catheter was 2.31±0.10. The total pregnancy rate was 44.8% (56/125) in Friedman IVF catheter and 43.6% (17/39) in Sydney IVF catheter. The difference was not significant (P>0.05) for age of patients, number of embryo transfer and total pregnancy rate between groups.

CONCLUSIONS:
Our study suggests that use soft catheters Sydney or Friedman of embryo transfer do not affect the outcome of ART cycles. This study was important because ET is probably the most important procedures in assisted reproduction and choice of the catheter is also very important for a good prognosis after embryo transfer.

Prolactin inhibits oocyte release after gonadotropin stimulation in the rat: putative mechanisminvolving ovarian production of beta-endorphin and prostaglandin

Polisseni F¹, Faletti AG, Pereira VM, Reis AM, Camargos AF, Reis FM.
FertilSteril. 2005 Apr;83Suppl 1:1119-24

Abstract

OBJECTIVE:
To evaluate whether prolactin (PRL) is able to inhibit ovulation induced with exogenous gonadotropins in the rat and whether this effect could be mediated by the ovarian production of beta-endorphin, prostaglandin, and nitric oxide (NO).

DESIGN:
Controlled in vivo and in vitro experiments.

SETTING:
Academic research laboratories.

ANIMAL(S):
Immature female rats undergoing ovulation induction with equine gonadotropins and hCG.

INTERVENTION(S):
Prolactin (100 or 200 microg), PRL + the opioid antagonist naloxone (200 microg each), or placebo were injected SC 4 hours after hCG administration for ovulation induction. In the in vitro experiments, isolated preovulatory ovaries were incubated with or without PRL in a final concentration of 100 or 200 ng/mL.

MAIN OUTCOME MEASURES(S):
Number of oocytes ovulated in vivo, ovarian beta-endorphin, PGE(2) and NO(2)(-)/NO(3)(-) release, and NO synthase activity in vitro.

RESULT(S):
Prolactin reduced significantly the number of oocytes ovulated at the doses of 100 and 200 microg, and this effect was partially reversed by naloxone administration together with 200 mug PRL. PRL also induced a twofold increase in the ovarian release of beta-endorphin and a threefold decrease in the ovarian production of PGE(2). Ovarian NO synthase activity and the concentrations of NO(2)(-)/NO(3)(-) in the incubation medium were not modified by PRL.

CONCLUSION(S):
Prolactin is able to reduce the number of oocytes released and modulate ovarian beta-endorphin and PGE(2) release, which may account for its peripheral anovulatory effects. This local effect of PRL could interfere in the process of ovulation induction by exogenous gonadotropins.

Clique aqui para o download da publicação

Depressão e ansiedade em mulheres climatéricas: fatores associados

POLISSENI, Álvaro Fernando et al. Rev. Bras. Ginecol. Obstet. [online]. 2009, vol.31, n.1, pp.28-34.ISSN 0100-7203. http://dx.doi.org/10.1590/S0100-72032009000100006.

OBJETIVO:
Determinar a prevalência de depressão e ansiedade em mulheres climatéricas e os prováveis fatores responsáveis por sua ocorrência.

MÉTODOS:
Em estudo transversal, foram selecionadas 93 mulheres que frequentaram um ambulatório de climatério no período de maio de 2006 a agosto de 2007. Como critério de inclusão foram consideradas mulheres na faixa etária de 40 a 65 anos e que concordaram em participar do projeto.

CRITÉRIOS DE EXCLUSÃO:
Pacientes em uso de terapia hormonal, hormonioterapia por implantes, DIUs e injetáveis de depósito nos últimos seis meses, endocrinopatias que levassem a irregularidades menstruais, hepatopatias, coagulopatias, uso de drogas que interferissem no ciclo menstrual, ansiolíticos e antidepressivos (pois o uso dessas drogas era indicativo de diagnóstico prévio de alterações do humor), histerectomizadas, ooforectomizadas, portadoras de câncer e de enfermidades psiquiátricas, pacientes que tivessem sido submetidas à radioterapia ou quimioterapia. Foram aplicados quatro questionários durante a entrevista: Anamnese, contendo dados sociodemográficos, clínicos e hábitos de vida; Índice Menopausal de Blatt-Kupperman, com o objetivo de diagnosticar as pacientes portadoras de síndrome climatérica; a subescala para Ansiedade, derivada da escala Hospitalar para Ansiedade e Depressão (HADS-A), com a finalidade de diagnosticar os casos de Ansiedade e o Inventário de Depressão de Beck, com o intuito de diagnosticar as mulheres portadoras de depressão. Foram realizadas as análises descritivas e de correlação entre as variáveis; o teste do χ2 e de Hosmer-Lemeshow, usando o programa Software Statistica versão 6.

RESULTADOS:
A média de prevalência de depressão entre as pacientes avaliadas foi de 36,8% enquanto que da ansiedade foi de 53,7%. Não houve diferença significativa entre a prevalência de depressão e ansiedade e as três fases do climatério. Observou-se relação significativa entre a presença de sintomas climatéricos de intensidade moderada e o aparecimento dessas alterações do humor (p<0,001). A depressão foi mais frequente em mulheres portadoras de ansiedade (OR=4,2) e insônia (OR=4,9) sendo a atividade remunerada considerada fator de proteção (OR=0,2). Os fatores de risco relacionados à ansiedade foram a presença de depressão (OR=6,1) e os antecedentes de tensão pré-menstrual (OR=7,0). CONCLUSÕES: a prevalência de depressão e ansiedade é elevada no climatério, sendo possível detectar fatores de risco relacionados à sua ocorrência.

Clique aqui para o download da publicação

A Successful Case of Genetic Pre-Implantation Study for Amiotrophic Lateral Sclerosis

Trabalho apresentado SBRA 2017
Polisseni, J., Assunção C. M. ; Polisseni, F., F., Rosa, J. V., Coutinho, L., Caetano, J. P.

INTRODUCTION
Pre-implantation genetic study case report (PGD) after ICSI in a patient with two parental cases of Amyotrophic Lateral Sclerosis (ALS), degenerative and progressive neurological disease, with motor neuron involvement.

METHODS
In June 2016, a 32-year-old patient underwent an ICSI cycle followed by PGD through STR and SNP-array (Karyomappings) and CGH-array (Comparative Genomic Hybridization by Microarray) binding.

RESULTS
In the ICSI cycle, 22 mature oocytes were obtained, with a fertilization rate of 95.5% (21/22) and blastocyst rate of 66.7% (14/21). All blastocysts were biopsied, frozen and the material sent for genetic analysis. First, nine embryos were analyzed, two of which presented a parental haplotype at risk for ALS disease. Then two embryos considered normal for ALS were submitted to the CGH-array and the two presented euploid, which were thawed and transferred to the patient in a new cycle. The patient had a twin pregnancy, uneventfully, with the birth of two healthy children at 32 weeks of gestation.

CONCLUSIONS
It was evidenced the relevance of the study of ALS through PGD post ICSI in patients with parental cases, since it is a disease related to genetic factors. The genetic diagnosis for monogenic disease was able to modify the risk of having children affected by ALS, since it still represents a serious disease, with no cure so far.

Avaliação da Eficiência das Técnicas de Reprodução Assistida (TRA’s) em Pacientes com Endometriose

Polisseni, J., Ribeiro, P. C., Polisseni, F., Rosa, J. V., Oliveira, N. F., Caetano, J. P.
Trabalho apresentado SBRA 2014

OBJETIVO
Avaliar a eficiência das TRA’s (qualidade embrionária e a taxa de gestação) em pacientes que tem exclusivamente endometriose como causa de infertilidade.

MÉTODOS
Estudo retrospectivo, desenvolvido sob aspecto descritivo, por meio de seleção de prontuários da clínica da Zona da Mata Mineira de 2001-2011. Incluiu-se no estudo apenas pacientes com endometriose como causa de infertilidade, faixa etária de 26 a 35 anos e que fizeram tratamento por Injeção Intracitoplasmática de Espermatozoide (ICSI) e Inseminação Intra-uterina (IIU). Os embriões foram avaliados e classificados de acordo com o protocolo da Rede Latino Americana de Reprodução Assistida. A taxa de gestação foi avaliada através da proporção de pacientes grávidas dentre o total de pacientes que foram submetidas às TRA´s, de cada grupo de estudo. Foi realizado teste de Qui-Quadrado, considerando p<0,05.

RESULTADOS
Avaliou-se 13 ciclos de IIU e 24 ciclos de ICSI. A idade média das pacientes foi de 32,3 anos. A taxa de fertilização na ICSI foi de 84,2% (273/324). Após a avaliação da qualidade embrionária 48 horas após a fertilização, observou-se que a maioria dos embriões encontrava-se no estágio de quatro células 51,7% (119/234), enquanto apenas 10,7% (25/234), 19,2% (47/234) e 18,4% (43/234) apresentavam, respectivamente duas, três e cinco ou mais células (p<0,05). 72 horas após a fertilização 60,7% (113/186) encontrava-se com 3-7 células, 26,9% (50/186) com oito células, enquanto 12,4% (23/186) com nove ou mais células. Não foi observada diferença significativa quando avaliado a qualidade embrionária dos embriões 48 e 72 horas (p = 0,326). A taxa de blastocisto foi de 35,3% (24/68). Não houve diferença significativa (p >0,05) quando comparados os dias de transferência embrionária e taxa de gestação. A taxa de gravidez clinica para ICSI foi de 41,7% (10/24), enquanto pela técnica de IIU foi de 7,7% (1/13), (p=0,031).

CONCLUSÃO
A endometriose não afetou a qualidade embrionária e taxa de gravidez clínica utilizando-se a técnica de ICSI. Entretanto, pacientes com endometriose que foram submetidas à IIU apresentaram resultado inferior ao esperado pelo método, nesta faixa etária de pacientes (15-20%), demonstrando que a ICSI parece ser mais eficiente para pacientes com endometriose independente do grau da doença.

Gene expression as biomarkers of quality to compare culture of system: single medium and sequential media system

Article in Fertility and Sterility 98(3):S167 · September 2012
DOI: 10.1016/j.fertnstert.2012.07.61

J. Polisseni, Polisseni, M. M. Pereira, M. O. Guerra,a L. S. A. Camargo, V. M. Peters.

OBJECTIVE
Evaluate embryo development and gene expression (HSP70.1, PRDX, GLUT-1 and GLUT-5) of blastocysts that cultured in two different systems: single medium, and a sequential media system, utilizing bovine embryo as a model. We compared the results obtained with medium utilized in bovine embryo: CR2aa and SOF.

DESIGN
Prospective randomized study.

MATERIALS AND METHODS
1040 cumulus oocyte-complexes were obtained from slaughterhouse ovaries, matured, and fertilized in vitro at 38.8C with 95% humidified air and 5% CO2. After, the presumptive zygotes were semi-denuned and were distributed randomly across the groups for cultured: CR2aa (n¼276), SOF (n¼264), seq€uencial media system: ECM/Multiblast – Irvine
(n¼163) and single medium Global – Life Global
(n¼284).
Cleavage and blastocyst ratewere evaluated. Real-time PCRwas used for gene expression analysis on the blastocysts. Statistical differences between treatment groups were analyzed using the Chi square test. The quantification of gene expression was analyzed with REST
as Pair Wise Fixed Reallocation Randomisation TEST
. P values<0.05 were considered significant. RESULTS: The evaluation of embryo development was not able to distinguish any difference of development amongst culture media from cleavage rate (72.1%, 76,1%, 74.0%, 76.4, respectively) thorugh blastocyst rate (30.4%, 27.0%, 35.0% and 38.0%, respectively) in CR2aa, SOF, ECM/Multiblast and Global, (P>0.05). Although, Global medium produced higher (P<0.01) expression of GLUT-1 and GLUT-5 compared with the ECM/multiblast. But neither sequencial and single medium were affect for expression of stress-responsive gene transcripts: HSP70.1 and PRDX.

CONCLUSION
Based on the embryo development the single media and sequential system was similar, but an expression of gene predicts that single media had a better performance because GLUTs was higher and energy metabolism
plays an important role during preimplantation embryo development and could be used as biomarkers for the study embryo viability.
Supported by: FAPEMIG.

Impact of the use of gonadotropins on embryonic development – a rat model.

Jornal Brasileiro de Reprodução Assistida (Impresso), v. 17, p. 32-35, 2013.
Autores: POLISSENI, Juliana; GRAZIA, J.G.V. ; GUERRA, M.O. ; MILEN, L.C. ; CAMARGO, L. S. A. ; PETERS, V. M.

ABSTRACT

Objective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control embryos.

Clique aqui para o download da publicação

Whole Genome Amplification on blastomers of post-biopsy bovine embryo.

POLISSENI, Juliana; MACHADO, Marco Antônio ; SOUZA, A. L. ; DOMINGUES, R. ; GUERRA, M. O. ; SERAPIÃO, Raquel Varella ; Pereira, M. M. ; SÁ, Wanderlei Ferreira de ; CARVALHO, B. C. ; VIANA, João Henrique Moreira ; PETERS, V. M. .

In: International Society of Embryos Transfer, 2009, California. Reproduction, Fertility and development, 2009. v. 01. p. 230-231

ABSTRACT
Biopsy of embryos is very useful for choosing the desired sex and for production of cloned and transgenic livestock. However, only a small amount of genomic DNA is available to perform genetic studies. Alternatively, methodologies using whole genome amplification (WGA) have been developed. The aims of this study were to evaluate the effect of WGA on blastomeres removed from 8- to 16-cell bovine embryos and to determine the sex of blastomeres. Oocytes obtained from slaughterhouse ovaries were in vitro matured and fertilized. On the fourth day after fertilization, 8- to 16-cell bovine embryos were biopsied, and one-fourth of an embryo was removed. The blastomeres (n = 56) were submitted to WGA followed by PCR. Prior to the whole genome amplification, male and female bovine DNA samples were serially diluted (30 ng μL–1, 3.0 ng μL–1, 0.3 ng μL–1, 0.03 ng μL–1, 0.003 ng μL–1, 0.0003 ng μL–1) and embryos of various development stages (2, n = 6; 4–7, n = 5; ≥8-cell, n = 5; blastocyst n = 27) were used to standardize PCR protocols and set the amplification limits. To digest the cellular cytoplasm and release the genomic DNA, embryos and blastomeres were submitted a 3 mg mL–1 proteinase K before PCR. Next, blastomeres were submitted to the GenomiPhi DNA Amplification Kit (GE Healthcare) according to manufacturer’s instructions. The product (1 μL) was electrophoresed on a 1% agarose gel stained with 3.0 μg mL–1 ethidium bromide. The reaction mixture was added to the material to be amplified (2 mm MgCl2, 5X PCR buffer, 0.2 mm each dNTPs, 0.05 U μL–1 GoTaq DNA polymerase, 0.25 μm of primer). The products were submitted to electrophoresis on 8% polyacrilamide gel and stained with silver nitrate procedure. The chi-square test was used for statistic evaluation of the results to test the WGA efficiency and to determine the sex rates of bovine embryos and biopsied samples submitted to PCR. It was possible to achieve 98% efficiency in amplifying blastomeres using the WGA kit. Amplified samples showed approximately 400 ng of DNA generated from an estimated initial amount of 12 pg of DNA resulting from two cells per embryo. In whole embryos from different stages, no diffference was detected in the proportion of sexes (P > 0.05). However, a greater number of female samples was noted in biopsied material (76%, 25/33) (P < 0.05). PCR efficiency in blastocysts (93%, 2/27) was statistically greater (P < 0.05) than embryos in early stages of development (83%, 5/6), and biopsied material to 2, 4–7 and ≥8-cell (40%, 2/5; 60%, 3/5; and 59%, 33/56; respectively). These differences could be related to a sex-chromosomal mosaicism or absence of a nucleus in biopsied samples. The WGA creates a DNA stock sample that could be used for various gene profiling and sex determination analyses.

Biopsy technique in bovine embryos produced in vitro at early stages of development: evaluation of quality and development capacity

POLISSENI, Juliana; GUERRA, M. O. ; SERAPIÃO, Raquel Varella ; Pereira, M. M. ; BOITE, M. C. ; CAMARGO, Luiz Sérgio de Almeida ; VIANA, João Henrique Moreira ; SÁ, Wanderlei Ferreira de ; PETERS, V. M.

In: Annual Conference of the International Embryo Transfer Society, 2008, Denver. Reproduction, 2008, Denver. Reproduction ,Fertility and Development, 2008. v. 20. p. 151-151

ABSTRACT
One of the causes of embryo mortality is chromosome abnormalities that occur during gametogenesis, fertilization, and embryo early development. Thus, a combination of morphological standards and techniques of molecular analyses could identify abnormal embryos. Preimplantation genetic diagnosis (PGD) is an emergent technology for use with farm animal embryos. With this procedure, blastomeres are removed by the biopsy of embryos at the 8- to 16-cell stage to provide cells for analyses of chromosome abnormalities prior to transfer. The aim of this study was to evaluate the effect of biopsy in bovine 8- to 16-cell embryos fertilized in vitro on embryo quality and subsequent development in vitro. A group of 706 oocytes were obtained from slaughterhouse ovaries, matured, and fertilized in vitro at 38.8°C with 95% humidified air and 5% CO2. The zygotes were semi-denuded and cultured in CR2aa medium under the same conditions as for in vitro fertilization. The rate of cleavage was 78.20%. Three days after fertilization, part of the 8- to 16-cell (298/706) embryos were distributed randomly across two groups: control (n = 103) and biopsy (n = 92) of blastomeres, and then returned to in vitro embryo culture to evaluate development until the blastocyst stage and the capacity to hatch. The amount of cells removed was one-fourth of the embryo. The blastocyst rate was evaluated on Day 8 after fertilization and the hatching rate on Day 10. Embryo morphology and quality were evaluated as previously described in the International Embryo Transfer Society manual (1998). To evaluate overall quality, embryos were stained on the 10th day of culture and the blastomeres were counted with the imaging software AxioVision 3.1 (Carl Zeiss, Feldbach, Switzerland). The blastocyst rate was analyzed by treatment groups with the chi-square test and the number of cells/embryo was analyzed by ANOVA with SAS (SAS Institute, Inc., Cary, NC, USA). The percentage of 8- to 16-cell embryos that developed to the blastocyst stage was similar (P > 0.05) between the control (66.0%, 68/103) and the biopsied (53.3%, 49/92) groups. Furthermore, no difference was noted in the hatching rates between the control group and the biopsied group (42.6%, 29/42 v. 44.9%, 22/49, respectively). Overall, no impact was detected on embryo quality from embryo biopsy with no difference in mean (±SE) blastocyst cell number between the control group (blastocysts: 67.1 ± 3.1; expanded blastocysts: 100.7 ± 6.9; hatched blastocysts: 189.9 ± 16.1) and the biopsied group (blastocysts: 61.1 ± 5.5; expanded blastocysts: 121.87 ± 10.6; hatched blastocysts: 187.3 ± 18.5). In conclusion, the biopsy used on 8- to 16-cell bovine IVF-derived bovine embryos does not affect the subsequent embryo development and number of cells/embryo or blastocyst, showing that it can be used to provide genetic material for preimplantation genetic diagnosis without affecting embryo quality. This work was supported financially by FAPEMIG.

Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis.

Fertility and Sterility, v. 93, p. 783-788, 2010
POLISSENI, JULIANA; SÁ, Wanderlei Ferreira de ; GUERRA, MARTHA DE OLIVEIRA ; MACHADO, Marco Antônio ; SERAPIÃO, Raquel Varella ; CARVALHO, BRUNO CAMPOS DE ; CAMARGO, Luiz Sérgio de Almeida ; PETERS, VERA MARIA

ABSTRACT

To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Randomized study. Molecular genetics and animal reproduction laboratories. Cow ovaries obtained from slaughterhouses. The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.

Clique aqui para o download da publicação

Effect of the pretreatment with prolactin on the distribution of immunoreactive beta-endorphinthrough different ovarian compartments in immature, superovulated rats

Reis FM1, Polisseni F, Pereira VM, Cassali GD, Reis AM, Faletti AG, Camargos AF.
J MolHistol. 2004 Nov;35(8-9):759-64.

ABSTRACT
Beta-endorphin and prolactin (PRL) are natural inhibitors of ovulation via central and peripheral mechanisms, but their possible interactions within the ovary are still unknown. The aims of the present study were to determine the gene expression and the topographic distribution of beta-endorphin, and the possible changes evoked by the pretreatment with PRL on the ovarian beta-endorphin localization in immature, superovulated rats. Prepuberal female Wistar rats weighing 60-70 g were superovulated with 20 IU equine gonadotrophins and, 48 h later, 20 IU human chorionic gonalotropin (hCG). Four hours after the hCG injection, the rats received either 200 microg rat PRL .i.p. (n = 12) or saline vehicle (n = 10). In the following morning the rats were killed and their ovaries were quickly removed. Beta-endorphin localization was assessed by immunohistochemistry and proopiomelanocortin (POMC) mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Beta-endorphin was expressed mostly in the corpora lutea and perivascular stroma, but a weak to moderate immunostaining was also present in the theca cells and some granulosa cells of tertiary/antral and preovulatory follicles. The main differences observed in the distribution of ovarian beta-endorphin between the two groups were a more intense immunostaining in the granulosa cells of antral follicles, corpus luteum and stroma of PRL-treated rats. POMC gene transcripts were detected in 2/5 samples from the control group and in 3/7 samples from the PRL-treated group. Thus, the expression of beta-endorphin in tertiary/antral follicles is enhanced by PRL treatment in immature, superovulated rats, providing a putative mechanism by which PRL could inhibit the ovarian response to induced ovulation.

Detection of chronic endometritis by diagnostic hysteroscopy in asymptomatic infertile patients.

Polisseni F¹, Bambirra EA, Camargos AF.
GynecolObstet Invest. 2003;55(4):205-10.

ABSTRACT
Chronic endometritis has been related to infertility and recurrent abortion. It is usually asymptomatic, and the diagnosis is rarely clinically suspected. We performed a prospective study to evaluate both the role of diagnostic hysteroscopy in the detection of chronic endometritis in infertile patients and Chlamydia trachomatis is a potential etiologic factor. Fifty consecutive patients who sought treatment for infertility in a tertiary academic hospital were submitted to diagnostic hysteroscopy and an endometrial biopsy for histopathological study and for diagnosis of C. trachomatis by polymerase chain reaction. The patients’ mean age was 33.7 +/- (SD) 5.4 years, and the duration of the couples’ infertility ranged from 1 to 18 years. The overall prevalence of chronic endometritis was 12% (6 patients). Among all patients, no cases of chlamydial infection were detected by polymerase chain reaction. In the detection of chronic endometritis, with 95% confidence intervals, the hysteroscopy sensitivity was 16.7% (range 0.9-63,5%), the specificity was 93.2% (range 80.3-98.2%), the positive predictive value was 25% (range 1.3-78.1%), and the negative predictive value was 89.1% (range 65.6-95.9%). These data suggest that hysteroscopy is not useful in the screening for chronic endometritis in asymptomatic infertile women. Further studies are needed to establish the etiology of endometritis in infertile patients.

Apoptosis modulation by activin A and follistatin in human endometrial stromal cells

Coutinho LM, et al. GynecolEndocrinol. 2016.

ABSTRACT
Activin A is a growth factor that stimulates decidualization and is abundantly expressed in endometrial proliferative disorders. Nevertheless, whether it directly affects endometrial cell survival is still unknown. This study investigated the effects of activinA on total death and apoptosis rates and on tumor necrosis factor (TNF) release by human endometrial stromal cells (HESC). We performed a controlled prospective in vitro study using primary HESC cultures obtained from healthy reproductive age women (n = 11). Cells were treated with medium alone (control) or activin A (25 ng/mL) or activin A (25 ng/mL) and its antagonist follistatin (250 ng/mL). Apoptosis and total cell death were measured by flow cytometry, while TNF concentrations in culture media were quantified by ELISA. Activin A decreased the percentage of apoptotic/dead cells from 31% to 22% (p < 0.05, paired t-test) and reduced TNF levels in culture medium by 14%, but there was no linear correlation between TNF release and apoptotic rates. Both effects of activin A were reversed by follistatin. These findings indicate that activin A promotes HESC survival, possibly by a TNF-independent pathway. This mechanism may be critical to the actions of activin A upon stromal cell growth and differentiation in physiology and disease.

Clique aqui para ver a publicação na íntegra 

Comparação entre diferentes métodos de separação de espermatozóides: swim up, Isolate e Percoll na viabilidade e produção in vitro de embriões bovinos

POLISSENI, Juliana; CARVALHO, B. C. ; Pereira, M. M. ; SERAPIÃO, Raquel Varella ; BATISTA, R. I. T. P. ; SILVA, M. R. ; BOITE, M. C. ; CAMARGO, Luiz Sérgio ; VIANA, João Henrique Moreira ; SÁ, Wanderlei Ferreira de ; IGUMA, L. T. In: 6 Congresso Internacional do Leite, 2007, Resende. 6 Congresso Internacional do Leite, 2007. p. 01-05.

Comparison in three diferents methods of sperm separation: swim up, isolate and percoll in the viability and bovine embryo in vitro produced

POLISSENI, Juliana; CARVALHO, B. C. ; Pereira, M. M. ; SERAPIÃO, Raquel Varella ; BATISTA, R. I. T. P.

In: 16th International Congress on Animal Reproduction, 2008, Budapeste. Abstract 16th International on Animal Reproduction, 2008.

Viabilidade da Técnica de biópsia em embriões bovinos fecundados in vitro em estádios iniciais do desenvolvimento

POLISSENI, Juliana; GUERRA, M. O. ; SERAPIÃO, Raquel Varella ; Pereira, M. M. ; FOLHADELLA, Isabella de Moura ; SÁ, Wanderlei Ferreira de ; VIANA, João Henrique Moreira ; BOITE, M. C. ; PETERS, V. M.

In: XXI Reunião Anual da SBTE, 2007, Costa do Sauípe. Acta Scientiae Veterinariae, 2007.. Porto Alegre, 2007.

Otimização do Protocolo de Polimerase Chain Reaction (PCR) para sexagem de embriões bovinos em diferentes estágios de Pré-implantação

POLISSENI, Juliana; CAMARGO, Luiz Sérgio de Almeida ; SÁ, Wanderlei Ferreira de ; MACHADO, Marco Antônio ; FERREIRA, Ademir de Moraes ; OLIVEIRA, Renata Sartini de ; RAMOS, Alessandra de Almeida.

Acta Scientiae Veterinariae, Porto Alegre, v. 32, n.1, p. 183, 2004.

Efeito do Nitroprussiato de Sódio sobre o desenvolvimento inicial de oócitos bovinos fecundados in vitro.

POLISSENI, Juliana; CAMARGO, Luiz Sérgio de Almeida ; SÁ, Wanderlei Ferreira de ; FERREIRA, Ademir Moraes ; VIANA, João Henrique Moreira ; OLIVEIRA, Renata Sartini de ; FRANCO, Gelsimara de Oliveira .

Acta Scientiae Veterinarie, Porto Alegre, v. 31, n.1, p. 528, 2003.

Influência dos fatores ambientais sobre a fertilidade masculina de pacientes do serviço de reprodução humana da zona da mata mineira.

POLISSENI, Juliana; POLISSENI, Fernanda ; RIBEIRO, P. C. ; ROSA, Josélio Vitoi ; FERREIRA, Newton ; CAETANO, J. P. J. .

In: XVI Congresso Brasileiro de Reprodução Assistida, 2012, Guarujá. Jornal Brasileiro de Reprodução Assistida. São Paulo, 2012. v. 16. p. 132-133.

Effect of maternal heat-stress on follicular growth and oocyte competence in Bos indicus cattle.

Theriogenology, v. 69, p. 155-166, 2008.

AUTORES: TORRES JÚNIOR, José Ribamar S ; Pires, M. F. A. ; SÁ, Wanderlei Ferreira de ; FERREIRA, Ademir de Moraes ; VIANA, João Henrique Moreira ; CAMARGO, Luiz Sérgio de Almeida ; RAMOS, Alessandra de Almeida ; FOLHADELLA, Isabella de Moura ; POLISSENI, Juliana ; Freitas, C.

Clique aqui para o download da publicação

Técnicas de Reprodução Assistida.

Boletim do Centro da Biologia da Reprodução, v. 25, p. 95-103, 2006.

Autores: POLISSENI, Juliana; GUERRA, M. O. ; SÁ, Wanderlei Ferreira de

Clique aqui para o download da publicação

Técnica de Tunel em Embriões de Ratas Wistar: Avaliação da Qualidade e da Capacidade de Desenvolvimento dos Embriões

Tunel technique in Wistar Rats Embryos: Evaluation of Qualify and Development Capacity of Embryos
Revista Interdisciplinar de Estudos Experimentais, v. 1, n. 3, p. 110 – 113, 2009

Autores: Juliana Polisseni, Raquel Varela Serapião, Paulo Henrique de Almeida Campos Júnior, João Gabriel Viana de Grázia, Larissa Cabral Milen, Luis Sérgio de Almeida Camargo, Martha de Oliveira Guerra, Vera Maria Peters

Clique aqui para o download da publicação

Efeito do transporte no desenvolvimento de embriões bovinos a fresco e reaquecidos após vitrificação.

Revista Brasileira de Zootecnia / Brazilian Journal of Animal Science, Viçosa, v. 35, p. 26-31, 2006.

AUTORES: RAMOS, Alessandra de Almeida ; POLISSENI, Juliana ; FERREIRA, Ademir de Moraes ; FOLHADELLA, Daniele da Silva ; SERAPIÃO, Raquel Varella ; CAMARGO, Luiz Sérgio de Almeida ; SÁ, Wanderlei Ferreira de ; NOGUEIRA, Luiz Altamiro Garcia

Clique aqui para o download da publicação

Influência da Azoospemia obstrutiva e não obstrutiva na eficiência da ICSI

Trabalho apresentado SBRA 2017
Polisseni, J., Assunção C. M. ; Polisseni, F., F., Rosa, J. V., Oliveira, N. F., Caetano, J. P.

OBJETIVOS
Avaliar a eficiência da ICSI em pacientes que foram submetidos à punção/biópsia testicular, possuindo causa obstrutiva (AO) e não-obstrutiva (ANO), como causa exclusiva de infertilidade. MATERIAL E MÉTODOS: Análise retrospectiva, pela seleção de prontuários da clínica da Zona da Mata Mineira de 2010-2017. A primeira parte do estudo incluiu-se apenas pacientes com azoospermia, que foram submetidos a punção/biópsia testicular seguida de ICSI. Avaliou-se o efeito da etiologia da azoospermia, comparando os ciclos de ICSI, entre AO (n=48) e ANO (n=28). A segunda parte focou-se no efeito da eficiência ICSI-AO-ANO comparado a ICSI de ejaculado a fresco (EF) (n=1006). Analisou-se taxas de fecundação, congelamento, gravidez clínica e aborto, através do teste de Qui-Quadrado, (p<0,05). RESULTADOS: Parte I: as taxas de fecundação, congelamento e gravidez clínica foram inferiores nos casos de ANO em relação à AO (75,2%, 25,9%, 39,3% vs 80,5%, 30,9%, 50,0%) e o grupo ANO apresentou maior taxa de aborto (18,2% vs 12,5%), sem significado estatístico. Parte II: os dados demonstraram resultados inferiores de taxa de fertilização para ANO quando comparado com EF (75,2% vs 83,7%), (p<0,05). Não
ocorreu diferença estatística entre AO, ANO e EF para taxas congelamento e aborto (p>0,05). Entretanto, AO apresentou taxa superior de gravidez clínica em relação à EF (50,0% vs 35,1%), p<0,05. CONCLUSÕES: Pressupõe-se que os ciclos de ICSI apresentaram melhor prognóstico em AO quando comparado a ANO e EF, por estar associada à normalidade do parênquima testicular e da espermatogênese.

A case report supporting the concept that some women have a predisposition for maternal meiosis erros

Polisseni, F.; Juliana Polisseni ;JosélioVitói Rosa ; Caetano, JP ; Coutinho, L.M. ; Carolina Marinho Assunção ; Jociane Viana Silva Oliveira
In: XX Congresso Brasileiro de Reprodução Assistida, 2016, Belo Horizonte. Abstracts of the 20th Annual Congress of the SBRA, Belo Horizonte/MG 14-17 September 2016, 2016. v. 20. p. 168-193.

The Impact of immatureoocyes quantity on the rates of fertilization, cleavag, embryo development and total pregnancy in ICSI cycles

Polisseni, Fernanda; Juliana Polisseni ;JosélioVitói Rosa ; CAETANO, JP ; Carolina Marinho Assunção ; COUTINHO, L. M. ; Jociane Viana Silva Oliveira
In: XX Congresso Brasileiro de Reprodução Assistida, 2016, Belo Horizonte. Abstracts of the 20th Annual Congress of the SBRA, Belo Horizonte/MG 14-17 September 2016, 2016. v. 20. p. 168-193.

Evaluation of pregnancy rate using two sot embryo transfer catheters

Polisseni, Fernanda; Juliana Polisseni ;JosélioVitói Rosa ; CAETANO, JP ; Carolina Marinho Assunção ; COUTINHO, L. M. ; Jociane Viana Silva Oliveira
In: Xx Congresso Brasileiro de Reprodução Assistida, 2016, Belo Horizonte. Abstracts of the 20th Annual Congress of the SBRA, Belo Horizonte/MG 14-17 September 2016, 2016. v. 20. p. 18-193.

Estudo da Viabilidade de Embriões Bovinos Pós-Biópsia: Modelo Experimental para Estudo da Técnica de Amplificação de Todo Genoma

Juliana Polisseni ; Polisseni, F. ; Wanderlei Ferreira de Sá ; Martha de Oliveira Guerra ; Vera Maria Peters
In: III Congresso internacional de Ginecologia e Obstetrícia de Minas Gerais, XXXIII Congresso Mineiro de Ginecologia e Obstetrícia, XIV Congresso de Ginecologia e Obstetrícia da Região Sudeste da FEBRASGO, 2008, Juiz de Fora. Revista Médica de Minas Gerais. Belo Horizonte: Coopmed, 2008. v. 18. p. 55-55.

Elective Embryo transfer after vitrification: a case series study

Polisseni, Fernandaet al.
In: XVIII Congresso Brasileiro de Reprodução Assistida, 2014, Salvador.
Abstracts of the Annual Congress of he SBRA, 2014. v. 18. p. 107-107.

Endometrial Scratching in Patients with Recurrent Implantation Failure: A case Series Study

Polisseni, Fernandaet al
In: XVIII Congresso Brasileiro de Reprodução Assistida, 2014, Salvador. Abstracts of the Annual Congress of he SBRA, 2014. v. 18. p. 106.

Gene expression as biomarkers of quality to compare culture of system: singlre medium and sequential medial system

Juliana Polisseni ; Polisseni, F. ; M M Pereira ; Martha de Oliveira Guerra ; Vera Maria Peters ; L S A Camargos
In: ASRM Annual Meeting, 2012, San Diego. ASRM Abstracts, 2012. v. 98. p. 167-167.

Prolactin inhibits oocyte release after gonadotropin stimulation in the rat: Putative mechanism involving ovarian production of beta-endorphin and prostaglandin

Polisseni, F.; Faletti, A. G. ; Pereira, M. V. ; Reis, M. A. ; Camargos, A. F. ; Reis, M. F
In: 12thI World Congress of Human Reproduction, 2005, Veneza. Anais World Congress on Human Reproduction, 2005.